Research Associate / Senior Research Associate, Protein Chemistry
TCR² Therapeutics Inc. is an immuno-oncology company developing novel cancer therapies that unleash the full signaling power of complete T cell receptors (TCR). TCR² has developed a unique proprietary TRuC™ platform which can reprogram the natural TCR complex to recognize specific antigens found on tumors where they elicit rapid killing of cancer cells. The company has demonstrated activity against both hematological and solid tumor targets in preclinical models and believes its TRuC™-reprogrammed T cells will serve as the engine for important therapies. TCR² was founded by MPM Capital and has scientific operations in Cambridge, MA to leverage best-in-class technologies and nucleate top-tier academic laboratories around the world.
The successful candidate will support the discovery, generation and characterization of antibody or single domain antibody (sdAb)-derived binders used in our proprietary TRuC™ platform. The position will work closely with experienced scientists in a dynamic, fast-paced and highly collaborative environment.
Identify and incorporate technologies that enhance our binder discovery capabilities
Support generation of phage display libraries, panning and screening campaigns
Design and clone protein expression constructs
Express proteins in bacterial and mammalian systems
Optimize purification using the AKTA platform
Use biochemical and biophysical methods to characterize antibodies and antigens
Support humanization and clone selection efforts
Measure protein binding by ELISA, Octet, and similar methods.
Perform epitope binning and mapping using Octet
Develop antigen specific cell lines and screen by flow cytometry
Support immunizations and screenings at CROs by providing QC-ed reagents
Run stability stability studies for protein and conjugates
B.S. or M.S. in molecular biology, biochemistry, protein chemistry or related discipline with a minimum of 4 years experience, preferably in a biotech or pharmaceutical setting
Experience with the design, generation and screening of phage display libraries
Experience in antibody discovery, e.g. engineering, expression, and/or characterization of antibodies, Fabs, scFvs, sdAbs and proteins in general
Expertise with protein expression systems (bacterial and mammalian e.g. CHO and HEK)
Extensive experience with protein purification systems (e.g. FPLC/AKTA) using affinity, SEC and ion exchange chromatograhy
Extensive experience with characterization of antibodies and proteins using biophysical and biochemical techniques (SDS-PAGE/Western blot, ELISA, SEC, Octet, epitope mapping and binning, etc.)
Experience in antibody humanization, antibody affinity maturation, and reformatting of antibodies is a plus but is not essential
The candidate should be a team player, highly organized with excellent verbal, written, and presentation skills, and also demonstrate scientific rigor and critical thinking
Interested candidates should send their resume to firstname.lastname@example.org