Scientist / Senior Scientist, Protein Engineering, Design and Selection

TCR² Therapeutics Inc. is an immuno-oncology company developing novel cancer therapies that unleash the full signaling power of complete T cell receptors (TCR). TCR² has developed a unique proprietary TRuC™ platform which can reprogram the natural TCR complex to recognize specific antigens found on tumors where they elicit rapid killing of cancer cells. The company has demonstrated activity against both hematological and solid tumor targets in preclinical models and believes its TRuC™-reprogrammed T cells will serve as the engine for important therapies.  TCR² was founded by MPM Capital and has scientific operations in Cambridge, MA to leverage best-in-class technologies and nucleate top-tier academic laboratories around the world.

The successful candidate will support the discovery and generation of antibody or single domain antibody (sdAb) derived binders used in our proprietary TRuC™ platform. The position will work closely with experienced scientists in a dynamic, fast-paced and highly collaborative environment.

Responsibilities:

  • Establish the design, generation and screening of phage display libraries for binder discovery in house

  • Design and clone protein expression constructs

  • Generate antibodies and antigens in bacterial and mammalian expression systems  

  • Use biochemical and biophysical methods to characterize sdAbs, scFvs and other protein scaffolds

  • Support clone selection, ranking, humanization and affinity maturation efforts 

  • Determine kinetic constants, epitope binning and mapping using Octet

  • Identify and incorporate technologies that enhance our binder discovery capabilities

Qualifications:

  • PhD in Molecular Biology, Biochemistry, Protein Chemistry or related discipline with a minimum of 4 years experience, preferably in a biotech or pharmaceutical settings

  • Extensive experience in total RNA extraction from PBMCs and lymphoid tissies, RNA QC, and cDNA library generation

  • Proficiency in phage display, screening and ranking (ELISA, Flow Cytometry, Octet) of binders

  • Genetic engineering, bacterial and mammalian expression systems, purification and characterization of binders and target antigens

  • Protein purification using AKTA FPLC by affinity, ion exchange and size exclusion chromatography

  • Characterization of proteins using biophysical and biochemical techniques - PAGE & Western blots, ELISA, Flow Cytometry, SEC, Octet, epitope binning and mapping

  • Humanization, affinity maturation, and reformatting of antibodies and sdAbs

  • The candidate should be a team player, highly organized with excellent communication skills, and demonstrate scientific rigor and excellent problem solving skills

Interested candidates should send their resume to careers@tcr2.com